己糖激酶/己糖磷酸激酶/HK/Hexokinase from Saccharomyces cerevisiae
发布时间:2012/8/30 14:33:16详细内容
英文名称:HK;Hexokinase from Saccharomyces cerevisiae;ATP:D-Hexose-6-phosphotransferase;Hexokinase from yeast
其他名称:己糖磷酸激酶
CAS号:9001-51-8
级别:BR
类型:Type F-300
分子量:~ 54 kDa (monomer);~110 kDa (dimer)
活力:≥130 units/mg protein (biuret)
活力定义:One unit will phosphorylate 1.0 μmole of D-glucose per min at pH 7.6 at 25℃
Optimal pH:7.5~9.0
Extinction Coefficient:E1% = 8.85 (PI) and 9.47 (PII) at 280 nm
Anion traces:sulfate (SO42-):free
Foreign activity:ATPase, myokinase, glucose-6-phosphate dehydrogenase, 6-phosphogluconic dehydrogenase and phosphoglucose isomerase ≤0.01%
性状:白色鳞状粉末。酿酒酵母提取。催化剂(Activators):Hexokinase requires Mg2+ ions (KM = 2.6 mM) for activity. Hexokinase is activated by catecholamines and related compounds;抑制剂(Inhibitors):sorbose-1-phosphate, polyphosphates, 6-deoxy-6-fluoroglucose, 2-C-hydroxy-methylglucose, xylose, lyxose, and thiol reactive compounds (Hg2+ and 4-chloromercuribenzoate)
用途:生化研究。为催化己糖6位上由ATP使之产生磷酸化反应的酶。对α-D葡萄糖的亲和性很高(Km=1×10-5M),对其他的己糖也可进行磷酸化。此反应的平衡向右偏移。△G°’=4.0千卡。该酶可被Mg2 、 K 所活化,但可被HS试剂阻抑。
保存:-20℃
更新时间:2024/4/1 14:39:57
标签:9001-51-8 试剂 化学试剂 生化试剂
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